Journal: Experimental and Therapeutic Medicine

Article Title: Naringenin inhibits migration of lung cancer cells via the inhibition of matrix metalloproteinases-2 and −9

doi: 10.3892/etm.2016.3994

Figure Lengend Snippet: Effect of naringenin on the MMP-2/MMP-9 activity and the expression of A549 cells. (A) A549 cells were treated with the indicated concentration of naringenin for 24 h. Conditional mediums were collected and MMP-2 and −9 activities were determined by gelatin zymography analysis. Lower panel: Data are presented as the mean ± standard deviation obtained from at least three independent experiments. The activity of the control was set to 1. *P<0.05 and **P<0.001 vs. control group (0 µM naringen). (B) RT-PCR analysis of MMP-2 and −9 expression. A549 cells were treated with naringenin for 24 h and the total RNA was extracted. The expression of MMP-2 and −9 was detected using a PCR assay using a 100 bp marker (in lane M). MMP, matrix metalloproteinase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; RT-PCR, reverse transcription-polymerase chain reaction.

Article Snippet: Total RNA (3 μg) was reverse transcribed to first strand cDNA using a Script Reverse Transcription System (Promega Corporation, Madison, WI, USA).

Techniques: Activity Assay, Expressing, Concentration Assay, Zymography, Standard Deviation, Control, Reverse Transcription Polymerase Chain Reaction, Marker, Reverse Transcription, Polymerase Chain Reaction